Comparative Study for Biosurfactant Production by Using Bacillus subtilis and Pseudomonas aeruginosa

Biosurfactants are amphiphilic compounds produced by various bacteria and fungi which reduce surface and interfacial tension. Oil contaminated soil were collected from four different automobile shop in Mayiladuthurai, Tamilnadu, India. Bacillus subtilis and Pseudomonas aeruginosa were isolated and identified from these four samples and these were screened for biosurfactants production using vegetable oil, kerosene, petrol and diesel by oil spreading technique and emulsification stability test and the best one of Bacillus subtilis and Pseudomonas aeruginosa were used for biosurfactants production using vegetable oil, kerosene, petrol and diesel as source. The isolated biosurfactants were identified using TLC method. Among four different oils; diesel is the best source for the production of biosurfatant in both Bacillus subtilis and Pseudomonas aeruginosa and Pseudomonas aeruginosa have higher activity than Bacillus subtilis.